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Determination of antihyperglycemic biguanides in serum and urine using an ion‐pair solid‐phase extraction technique followed by HPLC‐UV on a pentafluorophenylpropyl column and on an octadecyl column
Author(s) -
Tahara Kayoko,
Yonemoto Ayumi,
Yoshiyama Yuji,
Nakamura Toshiya,
Aizawa Masaaki,
Fujita Yoshikuni,
Nishikawa Takashi
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.685
Subject(s) - chromatography , chemistry , high performance liquid chromatography , solid phase extraction , urine , reagent , extraction (chemistry) , elution , biochemistry
An HPLC‐UV method was established for the determination of metformin and buformin in biological fluids. Metformin was not retained on particles packed in conventional solid‐phase extraction cartridges; in contrast, buformin was retained too firmly and not eluted with a solvent for recovery. However, both drugs were retained on particles that had been treated with an ion‐pair reagent of heptanesulfonate or dodecylsulfate and recovered almost completely. The recovered fraction was subjected to HPLC on a pentafluorophenylpropyl column which was suitable for the determination of both biguanides in serum and in urine. Limits of quantitation were low enough for clinical use, and reproducibility was high with an RSD of 0.9–2.3%. HPLC on a conventional octadecyl column was suitable only for the determination of buformin in serum since interfering peaks appeared on the chromatograms of urine samples. The method was applied to analysis of some clinical specimens. Copyright © 2006 John Wiley & Sons, Ltd.