Premium
Quantification of astaxanthin in shrimp waste hydrolysate by HPLC
Author(s) -
LópezCervantes J.,
SánchezMachado D. I.,
GutiérrezCoronado M. A.,
RíosVázquez N. J.
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.676
Subject(s) - chromatography , chemistry , astaxanthin , high performance liquid chromatography , hydrolysate , repeatability , methanol , shrimp , extraction (chemistry) , detection limit , fishery , carotenoid , biochemistry , organic chemistry , biology , hydrolysis
In the present study, a simple and rapid reversed‐phase HPLC method for the determination of astaxanthin in shrimp waste hydrolysate has been developed and validated. The analytical procedure involves the direct extraction of astaxanthin from the lipid fraction with methanol. The analytical column, SS Exil ODS, was operated at 25C. The mobile phase consisted of a mixture of water:methanol:dichloromethane:acetonitrile (4.5:28:22:45.5 v/v/v/v) at a flow rate of 1.0 mL/min. Detection and identification were performed using a photodiode array detector ( λ detection = 476 nm). The proposed HPLC method showed adequate linearity, repeatability and accuracy. Copyright © 2006 John Wiley & Sons, Ltd.