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Ascertainment of D ‐amino acids in germ‐free, gnotobiotic and normal laboratory rats
Author(s) -
Brückner Hans,
Schieber Andreas
Publication year - 2001
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.67
Subject(s) - chemistry , amino acid , feces , urine , chromatography , lactobacillus , biochemistry , enantiomer , microbiology and biotechnology , biology , stereochemistry , fermentation
Free D ‐amino acids were ascertained in the blood serum, urine and aqueous ethanolic extracts of feces of germ‐free laboratory rats and a rat made gnotobiotic (tetra‐associated) with species of Streptococcus, Lactobacillus and Clostridium . D ‐Amino acids were also determined in the brains of two germ‐free rats. For comparison, D ‐amino acids were also measured in the blood serum of normal rats and the blood plasma, urine and feces of normal white mice. D ‐Enantiomers of most protein L ‐amino acids were detected in all physiological samples of animals. Quantities of free D ‐amino acids were determined as N(O) ‐pentafluoropropionyl‐(2)‐propyl esters by enantioselective gas chromatography and mass spectrometry. Stereoisomers of the bacterial marker 2,6‐diaminopimelic acid, analyzed as N ‐trifluoroacetyl‐(2)‐propyl esters, were detected in feces of the gnotobiotic but not of the germ‐free rat. Copyright © 2001 John Wiley & Sons, Ltd. Abbreviations used: common protein amino acids are abbreviated according to three‐letter nomenclature Asx, Asp/AsnGlx Glu/GlnDap 2,6‐diaminopimelic acidGC‐SIM‐MS gas chromatographie/selected ion monitoring mass spectrometryOrn ornithin.
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