Premium
Method for derivatization of ephedrine and pseudoephedrine in nonaqueous media and determination by nonaqueous capillary electrophoresis with laser induced fluorescence detection
Author(s) -
Dong Yuming,
Chen Yonglei,
Chen Xingguo,
Hu Zhide
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.663
Subject(s) - chemistry , derivatization , ephedrine , capillary electrophoresis , chromatography , pseudoephedrine , laser induced fluorescence , fluorescence , mass spectrometry , pharmacology , medicine , physics , quantum mechanics
A nonaqueous capillary electrophoresis with laser‐induced fluorescence detection method was developed for the quantification of ephedrine and pseudoephedrine after derivatization with 4‐chloro‐7‐nitrobenzo‐2‐oxa‐1, 3‐diazol in nonaqueous media. The derivatization was made in off‐line mode. By a series of optimizations, a derivatization buffer composed of 40 mm ammonium acetate and 20% acetonitrile and a running buffer composed of 80 mm ammonium acetate and 3% acetic acid were applied for the derivatization and separation of ephedrine and pseudoephedrine, respectively. Linear relationships for ephedrine and pseudoephedrine were obtained in the range 1.23–19.60 mg/L (correlation coefficients 0.9970 for ephedrine and 0.9994 for pseudoephedrine), and the detection limits for ephedrine and pseudoephedrine were 0.014 and 0.011 mg/L, respectively. The method was applied to the analysis of ephedrine and pseudoephedrine in four preparations with recoveries in the range 93.9–105.1%. Copyright © 2006 John Wiley & Sons, Ltd.