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Liquid chromatographic/mass spectrometric assay of rabprazole in dog plasma for a pharmacokinetic study
Author(s) -
Feng Shao,
Guangji Wang,
Jianguo Sun,
Haitang Xie,
Hao Li,
Tian Lv,
Xiaoyan Zhu,
Jingwei Zhang
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.661
Subject(s) - chromatography , chemistry , pharmacokinetics , analyte , beagle , electrospray ionization , mass spectrometry , detection limit , electrospray , liquid chromatography–mass spectrometry , extraction (chemistry) , pharmacology , medicine
In order to evaluate the pharmacokinetic (PK) profile of rabeprazole (RA) sterile powder for injection, a rapid, sensitive and specific assay for quantitative determination of RA in dog plasma was developed and validated. After a liquid–liquid extraction procedure, samples were analyzed by liquid chromatography–electrospray ionization mass spectrometry (LC‐ESI‐MS) using omepazole as the internal standard (IS). The analyte and IS was chromatographed on a ZORBAX Extend‐C 18 analytical column (50 × 2 mm i.d, 5 µm, Agilent Technologies, USA). The assay was linear in the range 1–2000 ng/mL. The lower limit of quantification of RA was 1 ng/mL. The recovery of RA was greater than 70%. The within‐ and between‐batch accuracy was 102.7–107.4% and 103.5–105.7%, respectively. The plasma samples for the PK study were collected at defined time points during and after an intravenous injection (1 mg/kg) to beagle dogs and analyzed by LC‐ESI‐MS method. The PK parameters, such as half‐life, volume of distribution, total clearance and elimination rate constant, were determined. The PK profile of RA gave insights into the application in the clinics. Copyright © 2006 John Wiley & Sons, Ltd.