Screening and chromatographic assessing of a novel IgG biomimetic ligand | Zendy

Feng Hongqin | Zendy; Jia Lingyun | Zendy; Li Honglin | Zendy; Wang Xicheng | Zendy

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Screening and chromatographic assessing of a novel IgG biomimetic ligand

Author(s) -

Feng Hongqin,

Jia Lingyun,

Li Honglin,

Wang Xicheng

Publication year - 2006

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.654

Subject(s) - chemistry , chromatography , chromatographic separation , ligand (biochemistry) , biochemistry , high performance liquid chromatography , receptor

A novel biomimetic ligand, N‐ benzyloxycarbonyl‐ l ‐tyrosine ( N‐ cbz‐ l ‐Tyr), was screened by a combination method of molecular docking and immobilized receptor technique. Then, N‐ cbz‐ l ‐Tyr was immobilized on Sepharose CL‐4B to prepare a specific affinity adsorbent for immunoglobulin G (IgG). Scatchard analysis of the binding isotherm for IgG on the adsorbent gave an association constant ( K a ) of 4.91 × 10 6 m −1 and a theoretical maximum adsorption capacity of 17.3 mg IgG/mL gel. IgG with a purity of 98% was separated from human plasma by this new affinity adsorbent. Copyright © 2006 John Wiley & Sons, Ltd.

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