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Screening and chromatographic assessing of a novel IgG biomimetic ligand
Author(s) -
Feng Hongqin,
Jia Lingyun,
Li Honglin,
Wang Xicheng
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.654
Subject(s) - chemistry , chromatography , chromatographic separation , ligand (biochemistry) , biochemistry , high performance liquid chromatography , receptor
A novel biomimetic ligand, N‐ benzyloxycarbonyl‐ l ‐tyrosine ( N‐ cbz‐ l ‐Tyr), was screened by a combination method of molecular docking and immobilized receptor technique. Then, N‐ cbz‐ l ‐Tyr was immobilized on Sepharose CL‐4B to prepare a specific affinity adsorbent for immunoglobulin G (IgG). Scatchard analysis of the binding isotherm for IgG on the adsorbent gave an association constant ( K a ) of 4.91 × 10 6 m −1 and a theoretical maximum adsorption capacity of 17.3 mg IgG/mL gel. IgG with a purity of 98% was separated from human plasma by this new affinity adsorbent. Copyright © 2006 John Wiley & Sons, Ltd.