Determination of cocaine and cocaethylene in urine by solid‐phase microextraction and gas chromatography–mass spectrometry | Zendy

Yonamine Mauricio | Zendy; Saviano Alessandro Morais | Zendy

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Determination of cocaine and cocaethylene in urine by solid‐phase microextraction and gas chromatography–mass spectrometry

Author(s) -

Yonamine Mauricio,

Saviano Alessandro Morais

Publication year - 2006

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.643

Subject(s) - chemistry , chromatography , solid phase microextraction , mass spectrometry , gas chromatography–mass spectrometry , analyte , selected ion monitoring , polydimethylsiloxane , urine , gas chromatography , detection limit , sample preparation , biochemistry , organic chemistry

In order to evaluate recent cocaine exposure or its coingestion with ethanol, a simple and sensitive solid‐phase microextraction (SPME) procedure for determination of cocaine and cocaethylene in urine was developed and validated. A polydimethylsiloxane fibre (100 µm) was submersed in the urine sample for 20 min under magnetic stirring after alkalinization with solid buffer (NaHCO 3 :K 2 CO 3 , 2:1). Gas chromatography–mass spectrometry (GC‐MS) was used to identify and quantify the analytes in selected ion monitoring mode (SIM). The limits of quantification were 5.0 ng/mL for both analytes. Good inter‐ and intra‐assay precision was also observed (coefficient of variation <9%). Copyright © 2006 John Wiley & Sons, Ltd.

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