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Enantioselective high‐performance liquid chromatography of therapeutically relevant aminoalcohols as their fluorescent 1‐naphthyl isocyanate derivatives
Author(s) -
Ullrich Thomas,
Menge Sieglinde,
Schmid Martin,
Gübitz Gerald,
Krauss GerdJoachim
Publication year - 2001
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.64
Subject(s) - chemistry , derivatization , chromatography , isocyanate , enantiomer , high performance liquid chromatography , cellulose , urea , fluorescence , enantioselective synthesis , silica gel , organic chemistry , catalysis , polyurethane , quantum mechanics , physics
A method for determining the enantiomers of 10 therapeutically relevant aminoalcohols using HPLC and precolumn derivatization was developed. Naphthyl isocyanate reacted with racemic aminoalcohols to form urea derivatives which were separated isocratically on a cellulose tris(3,5‐dimethylphenylcarbamate) coated silica gel column, and detected fluorometrically in the lower ng mL −1 range. The effluents can also be monitored at lower sensitivity, using an ultraviolet detector operated at 220 nm. Copyright © 2001 John Wiley & Sons, Ltd.