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Determination of foscarnet (trisodium phosphonoformate) in pharmaceutical preparations by high‐performance liquid chromatography with ultraviolet detection
Author(s) -
García Javier,
Márquez Apolonia,
Ruiz Rosa,
López Luis F.,
Claro Carmen,
Lucero María Jesús
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.629
Subject(s) - chemistry , chromatography , detection limit , analyte , aqueous solution , chromatographic separation , coefficient of variation , relative standard deviation , high performance liquid chromatography , methanol , organic chemistry
An isocratic high‐performance liquid chromatographic method with UV detection has been developed and validated for the quantitative determination of foscarnet in isoosmotic sodium chloride aqueous solution. The mobile phase consisted of mixture of methanol:water (30:70 v/v), containing 1 m m tetrahexylammonium hydrogen sulphate at pH 5.80. The analyte was separated on a reversed‐phase C 18 column packed with 4 µm spherical particles of octadecylsilane. Hydrochlorothiazide was used as internal standard. UV detection at 232 nm allowed a quantification limit of 50 µg/mL. The assay was linear from 50 to 4000 µg/mL. The coefficient of variation was ≤2.52% for intra‐assay precision and ≤3.49% for inter‐assay precision. The deviation from the nominal value ranged from −0.57 to 0.47% for the same‐day accuracy and from −0.75 to 3.06% for day‐to‐day accuracy. Copyright © 2006 John Wiley & Sons, Ltd.