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Pharmacokinetic study of p ‐coumaric acid in mouse after oral administration of extract of Ananas comosus L. leaves
Author(s) -
Meng Zhen,
Wang Wei,
Xing DongMing,
Lei Fan,
Lan JiaQi,
Du LiJun
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.626
Subject(s) - chemistry , chromatography , pharmacokinetics , p coumaric acid , high performance liquid chromatography , oral administration , ananas , coefficient of variation , calibration curve , detection limit , pharmacology , ferulic acid , botany , biology
Quantification of p‐ coumaric acid in mouse plasma following oral administration of Ananas comosus L. leaves was achieved by reversed‐phase high‐performance liquid chromatography using a mobile phase of water–acetonitrile (82:18, v/v) and UV detection at 310 nm. The method was linear (determination coefficient, r 2 = 0.9997) within the tested range (0.04–1.28 µg/mL). Intra‐ and inter‐day precision coefficients of variation and accuracy bias were acceptable (maximal CV value was 4.06% for intra‐day and 4.19% for inter‐day) over the entire range. The recoveries were 90.63, 97.98 and 100.01% for concentrations of 0.04, 0.32 and 1.28 µg/mL, respectively. This is a very rapid, sensitive and economical way to determine p‐ coumaric acid concentration in mouse plasma after oral administration of A. comosus leaves. The concentration–time curve was fitted to the one‐compartment model. This is the first time that p‐ coumaric acid extracted from A. comosus leaves was detected by HPLC‐UV method and its pharmacokinetic characteristic was comprehensively studied. Copyright © 2006 John Wiley & Sons, Ltd.