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Determination of ligustilide in rat blood and tissues by capillary gas chromatography/mass spectrometry
Author(s) -
Shi Yunfeng,
He Langchong,
Wang Sicen
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.614
Subject(s) - chemistry , chromatography , detection limit , selected ion monitoring , mass spectrometry , gas chromatography–mass spectrometry , pharmacokinetics , calibration curve , quantitative analysis (chemistry) , gas chromatography , extraction (chemistry) , pharmacology , medicine
A gas chromatography/mass spectrometry (GC/MS) method was developed to study the pharmacokinetics of ligustilide following oral administration to rats. The method was used for the analysis of samples taken from rats. Biological samples were prepared by liquid–liquid extraction (LLE) using an n ‐hexane–ether (2:1) solvent mixture for a sample clean‐up step and analyzed by GC/MS with a quadrupole MS detector in selected ion monitoring mode ( m / z 190). The calibration curves were linear over the concentration range 0.172–8.60 µg/mL ( r > 0.99) for blood samples and a different range ( r > 0.99) for different tissue samples. The limit of detection (LOD) was 1.0 ng/mL or 1.0 ng/g (three times the signal–noise ratio). Within‐ and between‐day precision expressed as the relative standard deviation (RSD) for the method was 1.58–3.88 and 2.99–4.91%, respectively. The recovery for all samples was >80%, except for liver samples (>70%). The main pharmacokinetic parameters obtained were: T max = 0.65 ± 0.07 h, C max = 1.5 ± 0.2 µg/mL, AUC = 34 ± 6 h µg/mL and K a = 3.5 ± 0.6/h. The experimental results showed that ligustilide was easily absorbed, but its elimination was slow, from 3 to 12 h after oral administration. The concentrations of ligustilide in rat cerebellum, cerebrum, spleen and kidney were higher than those in other organs. Copyright © 2006 John Wiley & Sons, Ltd.