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Quantification of granisetron in human plasma by liquid chromatography coupled to electrospray tandem mass spectrometry
Author(s) -
Nirogi Ramakrishna V. S.,
Kandikere Vishwottam N.,
Shukla Manoj,
Mudigonda Koteshwara,
Maurya Santosh,
Boosi Ravikumar
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.613
Subject(s) - chromatography , chemistry , electrospray ionization , selected reaction monitoring , electrospray , mass spectrometry , analyte , tandem mass spectrometry , liquid chromatography–mass spectrometry , detection limit , bioequivalence , extraction (chemistry) , granisetron , high performance liquid chromatography , human plasma , pharmacokinetics , analytical chemistry (journal) , antiemetic , medicine , surgery , vomiting
A simple, sensitive and rapid high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry method was developed and validated for the assay of granisetron in human plasma. Following liquid–liquid extraction, the analytes were separated using an isocratic mobile phase on a reversed‐phase C 18 column and analyzed by MS in the multiple reaction monitoring mode using the respective [M+H] + ions, m/z 313/138 for granisetron and m/z 409/228 for the IS. The assay exhibited a linear dynamic range of 0.1–20 ng/mL for granisetron in human plasma. The lower limit of quantification was 100 pg/mL with a relative standard deviation of less than 5%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 2.0 min for each sample made it possible to analyze more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies. Copyright © 2006 John Wiley & Sons, Ltd.