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An HPLC/MS method for identifying major constituents in the hypocholesterolemic extracts of Chinese medicine formula ‘Xue‐Fu‐Zhu‐Yu decoction’
Author(s) -
Zhang HaiJiang,
Cheng YiYu
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.607
Subject(s) - decoction , chemistry , chromatography , traditional medicine , traditional chinese medicine , medicine , alternative medicine , pathology
In this paper, HPLC‐ESI‐MS technique was used to analyze the hypocholesterolemic extracts of ‘Xue‐Fu‐Zhu‐Yu decoction’, a traditional Chinese medicine consisting of six crude drugs (i.e. Paeonia lactiflora , Ligusticum chuanxiong , Citrus aurantium , Carthamus tinctorius , Prunus persica and Bupleurum falcatum ). A total of 17 compounds were identified and their plant derivations were also distinguished. Nine compounds among them were unambiguously determined as oxypaeoniflorin, amygdalin, albiflorin, paeoniflorin, ferulic acid, naringin, hesperidin, senkyunolide I and neohesperidin by comparing the retention times ( t R ), UV spectra and m / z values with those of the reference compounds. The other eight compounds were tentatively identified as prunasin, 6‐hydroxy‐kaempferol 3,6‐diglucoside, 6‐hydroxykaempferol 3‐rutinoside‐6‐glucoside, galloylpaeoniflorin, 6‐hydroxy‐kaempferol 3‐glucoside, anhydrosafflor yellow B, narirutin and kaempferol 3‐rutinoside by MS 2 spectra and the comparison of their UV spectra and MS spectra with literature data. Copyright © 2005 John Wiley & Sons, Ltd.