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High‐performance liquid chromatographic method for simple and rapid determination of linezolid in human plasma
Author(s) -
Boak Lauren M.,
Li Jian,
Nation Roger L.,
Rayner Craig R.
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.597
Subject(s) - chromatography , chemistry , linezolid , protein precipitation , high performance liquid chromatography , reproducibility , calibration curve , sample preparation , analyte , detection limit , bacteria , staphylococcus aureus , vancomycin , biology , genetics
A simple high‐performance liquid chromatographic (HPLC) method was developed and validated for rapid quantification of linezolid in human plasma. Protein precipitation using a mixture of 5% trichloroacetic acid and methanol (3:1, v/v) provided a straightforward method of sample preparation and the internal standard eperezolid was employed. A concentration range from 0.20 to 40.0 mg/L was utilized to construct calibration curves, and analysis of low‐ (0.40 mg/L), medium‐ (7.50 mg/L) and high‐quality (25.0 mg/L) control samples revealed excellent reproducibility (≤3.88%) and accuracy (≤4.20%). The recovery of both linezolid and eperezolid was approximately 100%. No interference was observed at the retention times of linezolid and eperezolid from blank plasma or eight commonly used antibiotics. Tests confirmed the stability of linezolid in plasma during three freeze–thaw cycles, on the bench during 24 h and during long‐term storage of frozen plasma for up to 12 weeks; in extracts it was stable in the HPLC autosampler over 12 h. Overall, this assay offers a highly simplistic approach to quantifying linezolid in plasma, and would be well suited to clinical pharmacokinetic, pharmacodynamic and toxicodynamic analyses. Copyright © 2005 John Wiley & Sons, Ltd.