Premium
Rapid determination of haloperidol and its metabolites in human plasma by HPLC using monolithic silica column and solid‐phase extraction
Author(s) -
AboulEnein Hassan Y.,
Ali Imran,
Hoenen Hubert
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.593
Subject(s) - chemistry , chromatography , human plasma , solid phase extraction , haloperidol , high performance liquid chromatography , extraction (chemistry) , silica gel , monolithic hplc column , column (typography) , plasma , phase (matter) , organic chemistry , dopamine , neuroscience , biology , physics , structural engineering , connection (principal bundle) , quantum mechanics , engineering
A rapid, sensitive and reproducible HPLC method was developed and validated for the analysis of haloperidol and its three main metabolites in human plasma. The analysis was carried out on a monolithic silica column (Chromolith Performance RP‐18e, 100 × 4.6 mm). The mobile phase consisted of sodium phosphate (0.1 m , pH 3.5)–acetonitrile (80:20, v/v) at a flow rate of 2.0 mL/min. UV detection at 230 nm was used, with the detection limits of these compounds ranging from 2 to 5 ng. The separation factors of all studied compounds were in the range 2.30–16.32, while the resolution factors were from 1.00 to 5.37. Copyright © 2005 John Wiley & Sons, Ltd.