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HPLC method for the determination and pharmacokinetic studies on geniposide in rat serum after oral administration of traditional Chinese medicinal preparation Yin‐Zhi‐Ku decoction
Author(s) -
Ye Guan,
Zhu HaiYan,
Zhao HaoLong,
Xu Bo,
Huang ChengGang
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.590
Subject(s) - chemistry , paeoniflorin , chromatography , decoction , pharmacokinetics , high performance liquid chromatography , analyte , calibration curve , solid phase extraction , oral administration , detection limit , pharmacology , traditional medicine , medicine
A new HPLC method for the determination of geniposide in rat serum with solid‐phase extraction (SPE) for preconcentration is described. Geniposide and an internal standard (paeoniflorin) were extracted from serum by SPE using C 18 cartridges. Analysis of the extract was then performed on a reversed‐phase C 18 column using acetonitrile–water (16:84, v/v) as the eluting solvent system, and UV detection at 238 nm was used to measure the analyte with a limit of quantitation about 0.1 µg/mL. The calibration curve for geniposide was linear ( r = 0.9993) in the concentration range 0.1–16.0 µg/mL. The intra‐ and inter‐day precision of the geniposide were determined and their RSD did not exceed 10%. The validated method has been successfully applied for pharmacokinetic studies of geniposide from rat serum after oral administration of Yin‐Zhi‐Ku decoction. Copyright © 2005 John Wiley & Sons, Ltd.