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Hybridation of different chiral separation techniques with ICP‐MS detection for the separation and determination of selenomethionine enantiomers: chiral speciation of selenized yeast
Author(s) -
Méndez Sonia Pérez,
González Elisa Blanco,
SanzMedel Alfredo
Publication year - 2001
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.59
Subject(s) - chromatography , chemistry , enantiomer , selenium , high performance liquid chromatography , chiral column chromatography , resolution (logic) , mass spectrometry , micellar electrokinetic chromatography , inductively coupled plasma mass spectrometry , detection limit , organic chemistry , artificial intelligence , computer science
Enantioseparation and determination of selenomethionine enantiomers in selenized yeast was investigated using chiral separation techniques based on different principles, coupled on‐line to inductively coupled plasma mass spectrometry (ICP‐MS) for selenium‐specific detection. High performance liquid chromatography (HPLC) on a β‐cyclodestrin (β‐CD) column, cyclodextrin‐modified micellar electrokinetic chromatography (CD‐MEKC), gas chromatography (GC) on a Chirasil‐L‐Val column, and HPLC on a Chirobiotic T column have been investigated as the chiral separation techniques. For HPLC separation on the β‐CD column, and also for CD‐MEKC, selenomethionine enantiomers were derivatized with NDA/CN − . For chiral separation by GC, selenomethionine enantiomers were converted into their N ‐trifluoroacetyl (TFA)‐ O ‐alkyl esters. The developed hybridation methodologies are compared with respect to enantioselectivity, sensitivity and analysis time. The usefulness of the best‐suited method [HPLC (Chirobiotic T)‐ICP‐MS] was demonstrated by its application to the successful chiral speciation of selenium and D ‐and L ‐selenomethionine content determination in selenized yeast. Copyright © 2001 John Wiley & Sons, Ltd.