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Screening for anabolic steroids in doping analysis by liquid chromatography/electrospray ion trap mass spectrometry
Author(s) -
Deventer K.,
Eenoo P. Van,
Delbeke F. T.
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.579
Subject(s) - chemistry , chromatography , electrospray , ion trap , anabolism , mass spectrometry , biochemistry
A fast and selective LC/MS/MS method for the screening of four anabolic steroids in human urine has been developed and validated. Liquid–liquid extraction with diethyl ether was applied after enzymatic hydrolysis. Analyses were performed on an ion trap mass spectrometer equipped with electrospray ionisation. MS/MS was applied for all compounds. The analytical run time was 11 min. The LOD for all compounds varied between 1 and 10 ng/mL. Left‐over A samples, which were declared positive by GC/MS for the presence of 3′‐hydroxystanozolol, were assessed using the described method. Copyright © 2005 John Wiley & Sons, Ltd.