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High‐performance liquid chromatographic method for simultaneous quantification of eight major biologically active ingredients in ‘Da‐Chai‐Hu‐Tang’ Preparation
Author(s) -
Li ChiaYing,
Chiu ChiungHui,
Huang HoShin,
Lin ChungHua,
Wu TianShung
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.572
Subject(s) - paeoniflorin , chromatography , baicalin , chemistry , emodin , high performance liquid chromatography , active ingredient , naringin , chai , pharmacology , medicine , philosophy , theology
A simple, sensitive and specific high‐performance liquid chromatographic method has been developed for the first time to simultaneously determine the eight major biologically active ingredients, namely paeoniflorin, naringin, sennoside A, baicalin, baicalein, saikosaponin a, rhein and emodin of the Chinese herbal formula Da‐Chai‐Hu‐Tang. The contents of these marker substances in Da‐Chai‐Hu‐Tang extract could be easily determined within 85 min. The assay was reproducible and accurate with overall intra‐day variations and accuracy of less than 2% and more than 97.9%, respectively. Results indicate that the developed HPLC assay can be successfully utilized as a quality control method for simultaneous determination of eight representative substances in Da‐Chai‐Hu‐Tang. Copyright © 2005 John Wiley & Sons, Ltd.