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Rapid liquid chromatography–tandem mass spectrometry method for quantification of ziprasidone in human plasma
Author(s) -
AlDirbashi Osama Y.,
AboulEnein Hassan Y.,
AlOdaib Ahmed,
Jacob Minnie,
Rashed Mohamed S.
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.571
Subject(s) - chromatography , chemistry , analyte , detection limit , calibration curve , mass spectrometry , coefficient of variation , ammonium acetate , liquid chromatography–mass spectrometry , tandem mass spectrometry , analytical chemistry (journal) , high performance liquid chromatography
A liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method for the determination of ziprasidone (ZIP) in human plasma was developed. ZIP and N ‐methyl ziprasidone as internal standard (IS) were extracted from alkalinized plasma using tert‐ butyl methyl ether. Separation was performed isocratically on a C8 column with 90% acetonitrile containing 2 mmol/L ammonium acetate as a mobile phase with a total run time of 2.5 min. MS/MS transitions of m / z 413 → 194 and m / z 427 → 177 of the analyte and internal standard were used for quantification. Confirmatory ions of m / z 413 → 177 and m / z 427 → 180 were collected as well. The calibration curve based on peak‐area ratio was linear up to at least 200 ng/mL with a detection limit of 0.1 ng/mL. The method showed satisfactory reproducibility with a coefficient of variation of less than 5%. The method was successfully applied to the analysis of ZIP in spiked human plasma. Copyright © 2005 John Wiley & Sons, Ltd.