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Determination of amino acid enantiomers in human urine and blood serum by gas chromatography–mass spectrometry
Author(s) -
Brückner Hans,
Schieber Andreas
Publication year - 2001
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.57
Subject(s) - chemistry , chromatography , urine , mass spectrometry , enantiomer , gas chromatography–mass spectrometry , gas chromatography , biochemistry , organic chemistry
Amino acid (AA) enantiomers were determined as N(O) ‐pentafluoropropionyl‐(2)‐propyl esters by chiral gas chromatography–mass spectrometry (GC‐MS) in 24 h samples of the urine of three healthy volunteers and in their blood sera. In urine the largest amounts were determined for D ‐Ser (64–199 µmol/day) and D ‐Ala (24–138 µmol/day). In blood sera, D ‐Ala (2.3–4.2 µmol/L) and D ‐Ser (1.0–2.9 µmol/L) were most abundant. Varying amounts of the D ‐enantiomers of Thr, Pro, Asx, Glx, Phe, Tyr, Orn and Lys were also found, albeit not in all urines and sera. Further, enantiomers were quantified in urine samples of two volunteers fasting for 115 h. Quantities of renally excreted D ‐AAs decreased in fasting, although amounts of D ‐Ser (69 and 77 µmol/L urine) as well as other D ‐AAs were still detectable. Time‐dependent analyses of urine showed that D ‐AAs are continuously excreted. Copyright © 2001 John Wiley & Sons, Ltd.