Premium
High‐performance liquid chromatographic assay for the active saponins from Panax notoginseng in rat tissues
Author(s) -
Li Lie,
Sheng YuXin,
Zhang JinLan,
Wang ShanShan,
Guo DeAn
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.567
Subject(s) - panax notoginseng , chemistry , chromatography , extraction (chemistry) , high performance liquid chromatography , calibration curve , kidney , quantitative analysis (chemistry) , saponin , detection limit , biology , pathology , medicine , alternative medicine , endocrinology
A reversed‐phase liquid chromatographic method was used to determine the ginsenosides Rg 1 , Rb 1 and Rd of Panax notoginseng in rat tissues (kidney, liver, heart, spleen and lung) after the administration of total saponins of P. notoginseng . The tissue samples were treated with solid‐phase extraction prior to HPLC. The calibration curves for the three saponins were linear in the given concentration ranges. The intra‐day and inter‐day assay coefficients in tissues were between 76 and 120% respectively. The recoveries of all the tissues were higher than 70%. This method was applied to evaluate the distribution of the three major saponins of P. notoginseng in rat tissues. Copyright © 2005 John Wiley & Sons, Ltd.