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Liquid chromatographic separation and UV determination of certain antihypertensive agents
Author(s) -
Bhushan R.,
Gupta Deepak,
Singh Shravan Kumar
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.557
Subject(s) - chromatography , chemistry , high performance liquid chromatography , amlodipine , solvent , methanol , column chromatography , chromatographic separation , extraction (chemistry) , medicine , organic chemistry , blood pressure , radiology
Abstract Three antihypertensive agents were extracted and isolated from commercial formulations. These were purified and characterized by melting point, λ max and IR. The percentage recovery by extraction process was in the range 81–91%. Active ingredients from binary formulations were separated by RP‐HPLC using methanol–water (50:50 v/v) and by TLC using CHCl 3 –CH 3 OH (6:1) as mobile phase. Detection was by UV at 210 nm in HPLC, and by iodine vapors in TLC. The solvent conditions from TLC were transferred to open column chromatographic separation. Quantitative determination was carried out using TLC and column chromatography supplemented with UV spectrophotometry. Recovery was in the range 82–93%. Two combination of drugs, viz. amlodipine + ramipril and amlodipine + enalapril, were separated by the three modes of liquid chromatography. The percentage recovery was in the range 80–92% by open column. Copyright © 2005 John Wiley & Sons, Ltd.