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Validated method for l ‐ornithine‐ l ‐aspartate analysis in human plasma by capillary electrophoresis
Author(s) -
Kowalski P.,
Bieniecki M.,
Olȩdzka I.,
Lamparczyk H.
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.551
Subject(s) - chemistry , chromatography , capillary electrophoresis , ornithine , electrophoresis , human plasma , biochemistry , amino acid , arginine
Abstract A rapid capillary electrophoresis method for routine determination of two amino acids, l‐ornithine and l‐aspartic acid, in human plasma is reported. The method runs automatically, requires a minimum of sample preparation and moreover includes no extensive extraction and no gradient or derivatization procedure. Analyses were performed on an uncoated silica capillary using buffer solution composed with 10 mm sodium tetraborate and 1 m sodium hydroxide (pH = 10.0). A capillary electrophoresis P/ACE system equipped with UV detection (200 nm), an automatic injector, a fluid cooled cartridge and System Gold data station was used in this study. The total analysis time under these conditions was 8.0 min. The calibration curve was linear in the range 10–280 µg mL −1 for l‐aspartic acid and 20–280 µg mL −1 for l‐ornithine (for both amino acids, r = 0.999). The method was validated by inaccuracy (bias) and precision (RSD) studies by analysing samples. The method was successfully applied to the quantitative determination of l‐ornithine‐l‐aspartate in human plasma and could be useful for clinical and bioavailability investigations. Copyright © 2005 John Wiley & Sons, Ltd.