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Direct resolution of (±)‐ephedrine and atropine into their enantiomers by impregnated TLC
Author(s) -
Bhushan R.,
Martens J.,
Arora Meenakshi
Publication year - 2001
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.55
Subject(s) - chemistry , ephedrine , chromatography , enantiomer , vapours , resolution (logic) , reagent , acetonitrile , silica gel , thin layer chromatography , tartaric acid , methanol , atropine , organic chemistry , artificial intelligence , neuroscience , computer science , citric acid , biology , medicine , anesthesia
Direct resolution of (±)‐ephedrine and atropine into their enantiomers was achieved by normal‐phase thin layer chromatography on silica gel plates impregnated with optically pure L ‐tartaric acid and L ‐histidine, respectively, as chiral selectors. The mobile phases enabling successful resolution were different combinations of acetonitrile–methanol–water. The spots were detected with iodine vapours and the detection limits were 2 and 6 µg, respectively, in terms of the racemate. The effects of concentration of the impregnating reagent, temperature and pH on resolution have been studied. Copyright © 2001 John Wiley & Sons, Ltd.