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Determination of paeoniflorin in rat plasma by a liquid chromatography‐tandem mass spectrometry method coupled with solid‐phase extraction
Author(s) -
Wang Qiao,
Yang Hanyu,
Liu Weina,
Feng Xiaolong,
Zhang Lantong,
Li Yanling,
Bi Kaishun,
Guo Dean
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.548
Subject(s) - paeoniflorin , chemistry , chromatography , electrospray ionization , formic acid , mass spectrometry , selected reaction monitoring , extraction (chemistry) , tandem mass spectrometry , analyte , detection limit , liquid chromatography–mass spectrometry , solid phase extraction , analytical chemistry (journal) , high performance liquid chromatography
A rapid, sensitive and selective liquid chromatography–tandem spectrometry method was developed and validated for determination of paeoniflorin in rat plasma using geniposide as the internal standard. The samples were pretreated with solid‐phase extraction using Extract‐Clean™ cartridges. Separation of paeoniflorin and IS was achieved on a reversed‐phase C 18 column (50 × 4.6 mm i.d.) with a mobile phase made up of acetonitrile and 0.05% formic acid (25:75, v/v) at a flow rate of 0.5 mL/min. Detection was carried out on a triple quadrupole tandem mass spectrometer by multiple‐reaction monitoring and an electrospray ionization source was employed as the ionization source. The lower limit of quantification obtained was 4 ng/mL ( n = 6) using 200 µL plasma with an accuracy of −3.67% (relative error) and a precision of 4.13% (relative standard deviation). A good linearity was found in the range of 4–1000 ng/mL. The intra‐ and inter‐day relative standard deviations in the measurement of quality control samples 10, 150 and 800 ng/mL ranged from 3.73 to 4.94% and from 4.31 to 6.56%, respectively. The accuracy was from −3.93 to −1.11% in terms of relative error. The analyte and IS were stable in the battery of stability studies. This method was successfully applied to a pharmacokinetic study of paeoniflorin after a single oral administration of 53.36 mg/kg paeoniflorin to rats. Copyright © 2005 John Wiley & Sons, Ltd.