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Validated HPLC method for the determination of fluconazole in human plasma
Author(s) -
Wattananat Triporn,
Akarawut Wiyada
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.538
Subject(s) - chromatography , chemistry , bioanalysis , human plasma , correlation coefficient , extraction (chemistry) , elution , coefficient of variation , methanol , high performance liquid chromatography , solid phase extraction , relative standard deviation , fluconazole , linear correlation , sample preparation , analytical chemistry (journal) , detection limit , antifungal , medicine , statistics , mathematics , organic chemistry , dermatology
A high‐performance liquid chromatographic assay with UV detection was developed for the determination of fluconazole in human plasma. The method utilized solid‐phase extraction for sample clean‐up. The separation was performed on a C 18 column by isocratic elution with a mobile phase of 10 m M acetate buffer at pH 5.0 and methanol and UV detection at 210 nm. Validation was performed according to the current recommendations of the USFDA bioanalytical method validation guidance. The method proved to be specific, accurate, precise and linear between 200 and 10,000 ng/mL with correlation coefficients greater than 0.999. The coefficient of variation was within 11% and relative deviation was less than 10%. Copyright © 2005 John Wiley & Sons, Ltd.