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Determination of rizatriptan in human plasma by liquid chromatographic–eletrospray tandem mass spectrometry: application to a pharmacokinetic study
Author(s) -
Guo Jifen,
Zhang Aijun,
Zhao Ling,
Sun Xiaohong,
Zhao Yimin,
Gao Hongzhi,
Liu Zeyuan,
Qiao Shanyi
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.528
Subject(s) - chromatography , chemistry , rizatriptan , mass spectrometry , analyte , tandem mass spectrometry , electrospray ionization , liquid chromatography–mass spectrometry , selected reaction monitoring , electrospray , pharmacokinetics , extraction (chemistry) , pharmacology , medicine , biochemistry , receptor , sumatriptan , agonist
Abstract A sensitive liquid chromatographic–tandem mass spectrometry(LC–MS[sol ]MS) method was developed for the determination of rizatriptan in human plasma. The analytes were extracted from plasma samples by liquid–liquid extraction, separated on a Zorbax XDB C 8 column (150 × 4.6 mm i.d.) and detected by tandem mass spectrometry with an electrospray ionization interface. Zomitriptan was used as the internal standard. The method had a lower limit of quantitation of 50 pg[sol ]mL for rizatriptan, which showed more sensitivity and speed of analysis compared with reported methods. The within‐ and between‐day precision was measured to be below 11.71% and accuracy between −5.87 and 0.86% for all quality control samples. This quantitation method was successfully applied to the evaluation of the pharmacokinetic profiles of rizatriptan after single oral administration of 5, 10 and 15 mg rizatriptan tablets to 10 healthy volunteers (five males and five females). Copyright © 2005 John Wiley & Sons, Ltd.