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Simultaneous determination of six HIV protease inhibitors (amprenavir, indinavir, lopinavir, nelfinavir, ritonavir and saquinavir), the active metabolite of nelfinavir (M8) and non‐nucleoside reverse transcriptase inhibitor (efavirenz) in human plasma by high‐performance liquid chromatography
Author(s) -
Hirabayashi Yoshihiro,
Tsuchiya Kiyoto,
Kimura Satoshi,
Oka Shinichi
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.521
Subject(s) - saquinavir , nelfinavir , amprenavir , indinavir , chemistry , ritonavir , lopinavir , efavirenz , virology , darunavir , active metabolite , metabolite , protease , human immunodeficiency virus (hiv) , enzyme , viral load , medicine , biochemistry , hiv 1 protease , antiretroviral therapy
We report the development of a simple, economical and reliable chromatographic method for the simultaneous determination of six HIV protease inhibitors (PIs; amprenavir, indinavir, lopinavir, nelfinavir, ritonavir and saquinavir), the active metabolite of nelfinavir (M8) and the non‐nucleoside reverse transcriptase inhibitor (NNRTI; efavirenz) in human plasma. After extraction from plasma with an ethyl acetate–acetonitrile mixture, the analytes were separated on a phenyl column with a gradient of acetonitrile and phosphate solutions, and detected at three ultraviolet wavelengths. Calibration curves were linear over the range 0.025–15 µg[sol ]mL for saquinavir and 0.05–15 µg[sol ]mL for the other analytes. The accuracies ranged from −6.9% to +7.6%, and the intra‐assay and inter‐assay precisions were <9.2 and <11.8%, respectively. Our method, covering most of the PIs and NNRTIs currently used, facilitates ready therapeutic drug monitoring in hospital laboratories. Copyright © 2005 John Wiley & Sons, Ltd.