Premium
A fast, cost‐saving and sensitive method for determination of cefuroxime in plasma by HPLC with ultraviolet detection
Author(s) -
Hernandis Verónica,
Escudero Elisa,
Pareja Ana,
Marín Pedro
Publication year - 2021
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.5188
Subject(s) - chromatography , chemistry , cefuroxime , trifluoroacetic acid , calibration curve , high performance liquid chromatography , pharmacokinetics , protein precipitation , detection limit , cephalosporin , therapeutic drug monitoring , antibiotics , pharmacology , medicine , biochemistry
Cefuroxime (CFX) is a broad‐spectrum second‐generation cephalosporin and one of the best choices for antibiotic prophylaxis. However, when used in critically ill patients, it may present changes in its pharmacokinetic properties. Therefore, therapeutic drug monitoring of CFX is necessary for effective dosing strategies. A simple, rapid and sensitive liquid chromatographic method with UV detection was developed and validated for the quantification of CFX in plasma. The method involved a single‐step precipitation of proteins with methanol and trifluoroacetic acid. Cefuroxime was analyzed on a Brisa LC 2 C 18 column in isocratic mode consisting of 0.1% trifluoroacetic acid in water and acetonitrile (75:25) with UV detection at a wavelength of 280 nm. The retention times of CFX and cephazolin (internal standard) were 9.8 and 7.4 min, respectively. The calibration curve was linear over a concentration range of 0.25–50 μg/ml. The limits of detection and quantification were 0.1 μg/ml and 0.25 μg/ml, respectively. The accuracy and precision were always <10%. The mean recovery was 93.52%. This fast and simple method could be applied in routine analysis and pharmacokinetic studies.