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Pharmacokinetic characteristics of hydroxysafflor yellow A in normal and diabetic cardiomyopathy mice
Author(s) -
Yao Rui,
Cao Yu,
Jiang Ruibin,
Zhang Xuan,
Li Feng,
Wang Siwang
Publication year - 2021
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.5173
Subject(s) - chemistry , malondialdehyde , pharmacokinetics , glutathione peroxidase , superoxide dismutase , antioxidant , pharmacology , glutathione , medicine , biochemistry , enzyme
Hydroxysafflor yellow A (HSYA), a major active water‐soluble component in Carthamus tinctorius L., is considered a potential antioxidant with protective effects against myocardial injury. However, its pharmacokinetic characteristics in normal and diabetic cardiomyopathy (DCM) mice remain unknown. This study was designed to investigate the differences in the pharmacokinetics of HSYA between normal and streptozotocin‐induced DCM mice. HSYA in the mouse plasma was quantified using LC–MS/MS. Compared with the normal group, the DCM group showed a significantly higher area under the curve (AUC (0– t ) , AUC (0–∞) ) value and peak plasma concentration, suggesting a higher uptake of HSYA in the DCM mice, and a significantly lower plasma clearance and apparent volume of distribution, suggesting slower elimination of HSYA in the DCM mice. The levels of serum superoxide dismutase and glutathione peroxidase were significantly higher, and malondialdehyde content was significantly lower in DCM mice than in normal mice, indicating the antioxidative stress effect of HSYA. Furthermore, the correlation analysis revealed that the serum HSYA content in the DCM mice significantly positively correlated with antioxidant enzyme levels. These results showed that the pharmacokinetics of HSYA changed significantly in the DCM mice, and this may improve the antioxidative stress effect of the drug.