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Simple method for the assay of clindamycin in human plasma by reversed‐phase high‐performance liquid chromatography with UV detector
Author(s) -
Cho SungHee,
Im HoTaek,
Park WanSu,
Ha YongHwa,
Choi YoungWook,
Lee KyungTae
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.517
Subject(s) - chromatography , chemistry , high performance liquid chromatography , protein precipitation , trichloroacetic acid , detection limit , quantitative analysis (chemistry)
A rapid and simple high‐performance liquid chromatography (HPLC) method was developed and validated for the quantification of clindamycin in human plasma. After precipitation with 50% trichloroacetic acid (TCA) containing the internal standard, propranolol, the analysis of the clindamycin level in the plasma samples was carried out using a reverse‐phase cyano (CN) column with ultraviolet detection (204 nm). The chromatographic separation was accomplished with an isocratic mobile phase consisting of acetonitrile–distilled water–7.6 mm tetramethylammonium chloride (TMA) (60:40:0.075, v[sol ]v[sol ]v), adjusted to pH 3.2. The proposed method was specific and sensitive with a lower limit of quantitation (LLOQ) of 0.2 µg[sol ]mL. This HPLC method was validated by examining the precision and accuracy for inter‐ and intraday analysis in the concentration range 0.2–20.0 µg[sol ]mL. The relative standard deviations (RSD) in the inter‐ and intraday validation were 6.1–14.9 and 6.0–16.1%, respectively. In the stability test, clindamycin was found to be stable in human plasma during the storage and assay procedure. The present HPLC method was applied to the analysis of samples taken up to 12 h after a single oral administration of clindamycin in healthy volunteers. Copyright © 2005 John Wiley & Sons, Ltd.