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Pharmacokinetic comparisons of six steroid saponins in rat plasma following oral administration of crude and stir‐fried Fructus Tribuli extracts by UHPLC–MS/MS
Author(s) -
Song Xiao,
Yuan Yaohui,
Wang Shuyue,
Sun Xiaochen,
Zhang Chao,
Gao Peng,
Shi Lei
Publication year - 2021
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.5151
Subject(s) - chemistry , formic acid , chromatography , pharmacokinetics , cmax , triple quadrupole mass spectrometer , ammonium formate , extraction (chemistry) , selected reaction monitoring , analyte , in vivo , oral administration , mass spectrometry , pharmacology , tandem mass spectrometry , medicine , microbiology and biotechnology , biology
Modern pharmacological studies have shown that Fructus Tribuli can improve sexual function and treat cardiovascular diseases. In this study, we focused on comparing the pharmacokinetics of crude Fructus Tribuli (CFT) and stir‐fried Fructus Tribuli (SFT) to further clarify the changes in chemical composition in vivo . The quantitation of six analytes was performed in a triple quadrupole mass spectrometer using the multiple reaction monitoring mode. Separation was performed on a Halo® C 18 column using 0.05% formic acid and 5 μmol/L sodium formate in water, and 0.05% formic acid and 5 μmol/L sodium formate in acetonitrile as the mobile phase. The selectivity, precision, accuracy, extraction recovery, matrix effect and stability of the method were fully validated. Compared with the crude group, the parameters C max and AUC 0– t of terrestroside B and terrestrosin K increased significantly ( P < 0.05), but the C max and AUC 0– t of polianthoside D, terrestrinin D, tribuluside A and terrestrosin D were decreased, terrestrosin D being especially decreased ( P < 0.05), after oral administration of SFT extract. These results showed that the developed method was suitable for pharmacokinetic analysis of the six steroid saponins of CFT and SFT in rat plasma, and can be used to facilitate future clinical studies.