Premium
Comparison of column performances in direct high‐performance liquid chromatographic enantioseparation of 1‐ or 3‐methyl‐substituted tetrahydroisoquinoline analogs. Application of direct and indirect methods
Author(s) -
Péter Antal,
Péter Mária,
Ilisz István,
Fülöp Ferenc
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.512
Subject(s) - chemistry , chromatography , isothiocyanate , cyclodextrin , derivatization , resolution (logic) , yield (engineering) , glycopeptide , teicoplanin , cellulose , high performance liquid chromatography , reagent , enantiomer , organic chemistry , vancomycin , materials science , artificial intelligence , biology , metallurgy , genetics , staphylococcus aureus , antibiotics , biochemistry , computer science , bacteria
The enantioseparability of 1‐ or 3‐methyl‐substituted tetrahydroisoquinolines was investigated by direct and indirect high‐performance liquid chromatography. α ‐ and β ‐cyclodextrin‐, macrocyclic glycopeptide‐ and cellulose‐based chiral columns and isothiocyanate‐ and Sanger‐type chiral derivatizing agents (CDAs) were applied to attain satisfactory enantioseparation. Of the chiral columns, β ‐cyclodextrin‐, vancomycin‐ and teicoplanin‐containing macrocyclic glycopeptide‐based columns appeared to be most suitable; of the CDAs, (1 S ,2 R )‐1‐acetoxy‐1‐phenyl‐2‐propyl isothiocyanate was most favorable. The differences between the selectivities of the columns and CDAs were compared and optimized to yield the best resolution. Copyright © 2005 John Wiley & Sons, Ltd.