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Qualitative, quantitative, and pharmacokinetic study on the absorbed components of Ardisia japonica ( Thunb .) Blume in rat plasma based on molecular networking combined with quadrupole time‐of‐flight LC/MS and triple quadrupole LC/MS
Author(s) -
Wang He Chen,
Li Tian Jiao,
Bao Yong Rui,
Wang Shuai,
Meng Xian Sheng
Publication year - 2021
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.5099
Subject(s) - chemistry , quadrupole time of flight , phytochemical , pharmacokinetics , chromatography , quantitative analysis (chemistry) , qualitative analysis , mass spectrometry , pharmacology , tandem mass spectrometry , biochemistry , medicine , qualitative research , social science , sociology
Isolation and screening of different compounds from plant extracts are always the key for natural drug research, and the absorbed prototype components have been considered as potential active ingredients. UHPLC combined with quadrupole time‐of‐flight mass spectrometry (Q‐TOF‐LC/MS) has been widely used in the research of natural drugs; however, we still need a more effective tool to compare and treat from a raw data. In this study, we provided a fast analytical method to measure the absorbed prototype components and their metabolites both qualitatively and quantitatively based on molecular networking (MN). For example, in Ardisia japonica ( Thunb .) Blume , a total of eight absorbed prototype components in rat plasma were identified. Furthermore, pharmacokinetic study was also successfully performed on the eight absorbed prototype components in rat plasma. Our findings have provided important information on the investigation of A. japonica in vivo . More importantly, the MS network analysis pattern serves as an integral solution for qualitative and quantitative determination of phytochemical compounds in natural drugs.