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The variation in the levels of 18 amino acids in the cortex and plasma of cerebral ischemia C57BL/6 mice
Author(s) -
Li JianSha,
Qiu Hongmei,
Jiang Qingsong,
Liu Yonghong,
Zhang Jing,
Gong Tao,
Jiang Xinhui
Publication year - 2021
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.5084
Subject(s) - chemistry , phenylalanine , glutamine , glycine , high performance liquid chromatography , leucine , amino acid , isoleucine , ischemia , cystine , glutamic acid , cortex (anatomy) , cerebral cortex , aspartic acid , biochemistry , medicine , chromatography , enzyme , cysteine , neuroscience , biology
Emerging evidence suggests that amino acid (AA) neurotransmitters play important roles in the pathophysiological processes of cerebral ischemia. In this work, an HPLC with fluorescence detection (HPLC‐FLR) method was developed for the simultaneous determination of 18 AAs in the cortex and plasma after cerebral ischemia in mice. The ischemia model was prepared by bilateral common carotid artery occlusion, and then the cortex and plasma of the sham, ischemia, and naringenin groups were collected. Based on the protein precipitation method, a simple and effective sample preparation method was developed. The treated sample contained minimal proteins and lipids. The analysis of the sample was performed by the proposed HPLC‐FLR method in combination with o ‐phthalaldehyde. The results showed a statistically significant increase in excitatory AAs (aspartic acid and glutamic acid), inhibitory AAs (glycine and 4‐aminobutyric acid), phenylalanine, citrulline, isoleucine, and leucine levels, and a decrease of glutathione and phenylalanine levels when compared with the sham group in the cortex. Besides, the administration of naringenin had significant effects on excitatory AAs, inhibitory AA (glycine), glutamine, tyrosine, phenylalanine, and leucine levels when compared with the sham group in the cortex. These findings could be utilized in studying and clarifying the mechanisms of ischemia.