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Metabolic profiles of Jin‐hong tablets in rats by ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry
Author(s) -
Liu LingXian,
Cao Liang,
Shi DanFeng,
Wang ZhenZhong,
Xiao Wei,
Yao XinSheng,
Li HaiBo,
Yu Yang
Publication year - 2021
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.5072
Subject(s) - chemistry , chromatography , glucuronidation , quadrupole time of flight , in vivo , sulfation , tandem mass spectrometry , mass spectrometry , hydroxylation , demethylation , metabolic pathway , metabolite , metabolism , biochemistry , microsome , enzyme , gene expression , microbiology and biotechnology , gene , dna methylation , biology
Jin‐hong tablets (JHTs), a well‐known traditional Chinese patent medicine (TCPM), have been effectively used for the treatment of chronic superficial gastritis (CSG). The metabolic profile of TCPMs is performed to determine their bioactive components. In this study, a five‐step strategy based on ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry and metabolynx™ software combined with mass defect filter technique was developed to delineate the metabolic profile of JHT in vivo . As a result, a total of 163 JHT‐related xenobiotics (38 prototypes and 125 metabolites) were identified or tentatively characterized in rat biological samples, and the phase I and II metabolism processes mainly included demethylation, hydroxylation, sulfation, and glucuronidation. In addition, after oral administration of JHT, a large amount of alkaloid‐related ingredients was detected in rat plasma samples, indicating that alkaloids may play an important role in the treatment of CSG with JHT. This study is beneficial for understanding the JHT’s in vivo metabolic profiles and characteristics, which helps to reveal its in vivo effective components and provides a solid basis for further studies on its functional mechanism.