Premium
The effect of stationary phase on lipophilicity determination of β ‐blockers using reverse‐phase chromatographic systems
Author(s) -
Welerowicz Tomasz,
Buszewski Boguslaw
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.507
Subject(s) - lipophilicity , chemistry , acetonitrile , chromatography , methanol , ammonium acetate , phase (matter) , high performance liquid chromatography , reversed phase chromatography , silica gel , capacity factor , polar , analytical chemistry (journal) , organic chemistry , physics , astronomy
Evaluation of lipophilicity parameters for basic compounds using different chromatographic stationary phases is presented. An HPLC method for determination of lipophilic molecule–stationary phase interactions was based on gradient analysis. Differences in correlation between the lipophilicity of compounds and experimental chromatographic results obtained in pseudo‐membrane systems showed a strong influence of stationary phase structure and physico‐chemical properties. β ‐Blocker drugs with varying lipophilicity and bio‐activity were chosen as test compounds. The stationary phases used for the study were monolithic rod‐structure C 18 and silica gel octadecyl phase SG‐C 18 as reference material. The second group was silica gel‐based polar‐embedded alkylamide and cholesterolic phases. The mobile phase was composed of acetonitrile or methanol with ammonium acetate, and a linear gradient of methanol and acetonitrile in mobile phase was performed. A linear correlation of plots of log k g = f (log P ) was observed, especially for polar‐embedded phases, and this allowed log P HPLC to be calculated. The behavior of stationary phases in methanol and acetonitrile buffer showed differences between obtained log P HPLC values. Copyright © 2005 John Wiley & Sons, Ltd.