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Determination of dexamethasone acetate in CETETH 20‐based in liquid crystalline systems using HPLC
Author(s) -
Oyafuso Márcia Helena,
FonsecaSantos Bruno,
Kogawa Ana Carolina,
Carvalho Flávia Chiva,
Gremião Maria Palmira Daflon,
Chorilli Marlus
Publication year - 2021
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.5054
Subject(s) - chromatography , chemistry , high performance liquid chromatography , acetonitrile , context (archaeology) , dexamethasone , detection limit , relative standard deviation , methanol , phosphate buffered saline , organic chemistry , medicine , paleontology , endocrinology , biology
Dexamethasone acetate (DEX), a potent anti‐inflammatory, is used primarily in the treatment of inflammatory and autoimmune diseases. It was incorporated in CETETH 20 (polyoxyethylene 20 cetyl alcohol)–based liquid crystalline systems to enhance the purpose of the drug. Concomitant with the pharmaceutical technology performed, a HPLC method was developed and validated for the quantification of dexamethasone acetate in CETETH 20‐based liquid crystalline systems for the evaluation of the drug in the new matrix. The method was performed using a C18 column with acetonitrile:methanol:water (35:35:30, v/v/v) as the mobile phase at a flow rate of 0.8 mL min −1 at 239 nm. The method was linear in the range of 1–25 μg mL −1 ; the limit of quantification and limit of detection were 0.05 and 0.16 μg mL −1 , respectively; the accuracy of the method was 99.92% (relative standard deviation < 1%), and it presented intra‐day and inter‐day precision with deviations less than 1%. In this context, the method was successfully used to determine the incorporation efficiency of DEX in CETETH 20‐based liquid crystalline systems and can be easily used by pharmaceutical companies and laboratories around the world.