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A rapid and sensitive ultra‐high‐pressure liquid chromatography–tandem mass spectrometry method for the determination of notoginsenoside Ft1 in rat plasma with application to pharmacokinetic study
Author(s) -
Ju Zhengcai,
He Chunyong,
Li Jia,
Yang Li,
Wang Zhengtao
Publication year - 2021
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.5042
Subject(s) - chemistry , chromatography , protein precipitation , pharmacokinetics , panax notoginseng , tandem mass spectrometry , electrospray ionization , selected reaction monitoring , mass spectrometry , electrospray , liquid chromatography–mass spectrometry , detection limit , analyte , elution , matrix (chemical analysis) , pharmacology , medicine , alternative medicine , pathology
Notoginsenoside Ft1 (NGFt1), a dammarane triterpene glycoside isolated from Panax notoginseng , showed potent effective in stimulating platelet aggregation in our previous assay, yet its pharmacokinetic behavior is still unclear. This study describes a rapid and sensitive ultra‐high‐pressure LC–tandem mass spectrometry assay for determining of NGFt1 in rat plasma. Methanol‐mediated precipitation was used for sample pre‐treatment. Chromatographic separation was achieved on a C 18 column with gradient elution using water and acetonitrile as mobile phase. Determination was obtained using an electrospray ionization source in negative selected reaction monitoring (SRM) mode at the transitions of m/z 915.9 → m/z 783.8 and m/z 799.8 → m/z 637.8 for NGFt1 and internal standard, respectively. The assay was linear over the concentration range 0.25–2500 ng/mL ( r > 0.995) with the lower limit of quantification of 0.25 ng/mL. The intra‐ and inter‐day precisions (relative standard deviation, %) ranged 1.65%–9.84% and 2.46%–13.49%, respectively, whereas accuracy (relative recovery, %) ranged from 96.21% to 99.45%, respectively. The recovery ranged from 95.09% to 102.22% and the matrix effect from 98.29% to 100.13%. The analyte was stable under tested storage conditions. The method has been successfully applied to a preclinical pharmacokinetic study in rats after a single intravenous (2 mg/kg) and oral (50 mg/kg) administration.