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The exploration of neuraminidase inhibitory activity on Fallopia denticulata , an ethnic herb in China
Author(s) -
Zheng Yan,
Zhang Xiang,
Cao Yu,
Huang Linfang
Publication year - 2021
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.5024
Subject(s) - chemistry , coumarin , gallic acid , ethyl acetate , syringic acid , catechin , methyl gallate , herb , coptis chinensis , neuraminidase , polyphenol , chrysin , traditional medicine , chromatography , biochemistry , enzyme , medicinal herbs , flavonoid , antioxidant , organic chemistry , traditional chinese medicine , medicine , alternative medicine , pathology
This study was designed to explore the bioactive ingredients in the extracts of Fallopia denticulata (C.C. Huang) Holub, a medicinal plant grown in China, which exhibits the best neuraminidase (NA) inhibition activity. Three fractions of ethyl acetate, ethanol, and water were tested on NA inhibition assay, and the best one was conducted by ultra‐performance liquid chromatography–time‐of‐flight mass spectrometry in the negative and positive modes to analyze the metabolic components. The results revealed the identification of the following 21 compounds: 3 organic acids, 11 flavonoids, 1 coumarin, and 6 others, such as β‐daucosterol, gallic acid, and syringic acid, of which 12 compounds were discovered for the first time in F. denticulata . In addition, we used the molecular docking technique to support the anti‐NA activity of each compound in the best extract. The results confirmed that the two better bioactive compounds were (−)‐epicatechin gallate and (+)‐catechin. Therefore, F. denticulata could be used as a potential material for new anti‐influenza drugs.