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An LC–ESI/MS/MS method for the determination of lupeol via precolumn derivatization and its application to pharmacokinetic studies in rat plasma
Author(s) -
Wang Ziming,
Wang Yu,
Yu Tao,
Hu Zhiwei,
Wang Yang
Publication year - 2021
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.5005
Subject(s) - chemistry , lupeol , chromatography , derivatization , electrospray ionization , selected reaction monitoring , tandem mass spectrometry , mass spectrometry , liquid chromatography–mass spectrometry , organic chemistry
Lupeol, a phytosterol and triterpene, is widely found in edible fruits and vegetables, and has been reported to exhibit a spectrum of pharmacological activities against various disease conditions. In the present study, a derivative generated by the reaction of lupeol with p ‐toluenesulfonyl isocyanate was ionizable and fragmentable in the negative mode by electrospray ionization/tandem mass spectrometry. Based on this simple chemical derivatization, a liquid chromatography–electrospray ionization/tandem mass spectrometry method was developed and validated for the quantification of lupeol in rat plasma. The calibration curves were linear ( r 2 > 0.999) over concentrations from 2.5 to 250 ng/ml for lupeol. The method had an accuracy of 96.0–109.4%, and the intra‐ and inter‐day precisions (RSD) were within ± 15%. The stability data showed that no significant degradation occurred under the experimental conditions. The mean recoveries at three quality control levels were within 88.7–95.7%. No significant matrix effects (105.3–109.8%) were observed in rat plasma. This method was successfully applied to the pharmacokinetic study of lupeol in rat plasma after oral administration.