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Rapid determination of rosmarinic acid and its two bioactive metabolites in the plasma of rats by LC–MS/MS and application to a pharmacokinetics study
Author(s) -
Wang Xiangyang,
Qian Yuexin,
Li Xue,
Jia Xue,
Yan Zhexuan,
Han Min,
Qiao Miao,
Ma Xiaohui,
Chu Yang,
Zhou Shuiping,
Yang Wenzhi
Publication year - 2021
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4984
Subject(s) - rosmarinic acid , chemistry , chromatography , pharmacokinetics , caffeic acid , oral administration , ferulic acid , metabolite , tandem mass spectrometry , in vivo , liquid chromatography–mass spectrometry , high performance liquid chromatography , mass spectrometry , pharmacology , biochemistry , medicine , microbiology and biotechnology , biology , antioxidant
Rosmarinic acid (RA), an ester compound of caffeic acid (CA) and 3,4‐dihydroxyphenyllacic acid, is widely distributed in the herbs of the Lamiaceae family and has shown a wide spectrum of pharmacological properties. CA and FA (ferulic acid) are two bioactive metabolites in vivo after oral administration of RA; however, a rapid and robust analytical approach that can enable the quantitative assay of RA and two bioactive metabolites is still lacking. A liquid chromatography/tandem mass spectrometry method was established that was capable of the quantitative determination of RA, CA and FA by negative‐mode multiple reaction monitoring within 7 min using a Zorbax SB‐C 18 column and an isocratic elution. This assay method was validated as linear over the investigated ranges with correlation coefficients ( r ) > 0.9950. The intra‐ and inter‐day precision was <10.65%, and the accuracies (relative error, %) <−6.41%. The validated approach was applied to a pharmacokinetics study of RA and its two metabolites in rats after oral and intravenous administration. RA was rapidly metabolized in both administration modes, whilst the metabolites CA and FA were only detectable by oral administration. The absolute availability of RA was calculated to be 4.13%.