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Rapid, simple and highly sensitive LC‐ESI‐MS/MS method for the quantification of tamsulosin in human plasma
Author(s) -
Ramakrish. V. S.,
Vishwottam K. N.,
Manoj S.,
Koteshwara M.,
Wishu S.,
Varma D. P.
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.498
Subject(s) - chemistry , chromatography , analyte , protein precipitation , tamsulosin , mass spectrometry , detection limit , formic acid , electrospray ionization , liquid chromatography–mass spectrometry , extraction (chemistry) , selected reaction monitoring , sample preparation , tandem mass spectrometry , medicine , pathology , hyperplasia
A simple, rapid, sensitive and specific liquid chromatography–tandem mass spectrometry method was developed and validated for quantification of tamsulosin (I), a highly selective α 1‐adrenoceptor antagonist used for the treatment of patients with symptomatic benign prostatic hyperplasia. The analyte and internal standard, mosapride (II) were extracted by liquid–liquid extraction with diethyl ether‐dichloromethane (70:30, v[sol ]v) using a Glas‐Col Multi‐Pulse Vortexer. The chromatographic separation was performed on a reverse phase Waters symmetry C 18 column with a mobile phase of 0.03% formic acid–acetonitrile (30:70, v[sol ]v). The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The mass transitions m[sol ]z 409.1 ⊘ 228.1 and m[sol ]z 422.3 ⊘ 198.3 were used to measure I and II, respectively. The assay exhibited a linear dynamic range of 0.1–50.0 ng[sol ]mL for tamsulosin in human plasma. The lower limit of quantitation was 100 pg[sol ]mL with a relative standard deviation of less than 10%. Acceptable precision and accuracy were obtained for concentrations over the standard curve ranges. A run time of 2.0 min for each sample made it possible to analyze a throughput of more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies. Copyright © 2005 John Wiley & Sons, Ltd.