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Application of an LC–ESI–QTOF–MS method for evaluating clindamycin concentrations in plasma and prostate microdialysate of rats
Author(s) -
Ritter Lisiani,
Possa Eduarda,
Bergoza Larissa,
Agostini Fabiana,
Silva Sidnei M.,
Tasso Leandro
Publication year - 2020
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4977
Subject(s) - chemistry , chromatography , clindamycin , microdialysis , electrospray ionization , formic acid , prostate , mass spectrometry , antibiotics , medicine , biochemistry , extracellular , cancer
Clindamycin is used for infections caused by Gram‐positive and Gram‐negative anaerobic pathogens and Gram‐positive aerobes. Propionibacterium acnes is an important opportunistic microorganism of the human skin and is related to prostatitis. An LC–electrospray ionization–quadrupole time‐of‐flight–MS method was validated for determining clindamycin concentrations in plasma and prostate microdialysate. Clindamycin separation was carried out on a C 18 column at 0.5 mL/min. The mobile phase employed gradient elution of formic acid and methanol. A mass spectrometer was operated in positive electrospray ionization mode to monitor ion 425.1784 and 253.1152 for clindamycin and cimetidine (internal standard), respectively. Linearity was obtained at 0.5–10.0 μg/mL (plasma) and 0.05–1.0 μg/mL (microdialysate) with coefficients of determination ≥0.999. The intra‐ and inter‐day precision (coefficient of variation ‐ CV%) values were ≤13.83% and 12.51% for plasma, respectively, and ≤10.90% and 9.35% for microdialysate, respectively. The accuracy was between 90.82% and 108.25% for plasma, and 96.97% and 106.98% for microdialysate. The present method was fully validated and applied to investigate clindamycin concentrations in both plasma and prostate by microdialysis in Wistar rats (80 mg/kg, intravenous). Because the penetration of antibiotics into the prostate may be restricted, this method allows us to investigate the prostate concentrations of clindamycin for the first time.