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Detection of androst‐4‐ene‐3,6,17‐trione (6‐OXO ® ) and its metabolites in urine by gas chromatography–mass spectrometry in relation to doping analysis
Author(s) -
Van Thuyne W.,
Van Eenoo P.,
Mikulčíková P.,
Deventer K.,
Delbeke F. T.
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.496
Subject(s) - chemistry , chromatography , urine , steroid , detection limit , ene reaction , gas chromatography–mass spectrometry , mass spectrometry , reagent , gas chromatography , nandrolone , anabolic steroid , selected ion monitoring , ether , anabolism , organic chemistry , hormone , biochemistry
The metabolism and excretion of androst‐4‐ene‐3,6,17‐trione after administration of the ‘nutritional’ supplement 6‐OXO ® was investigated by gas chromatography–mass spectrometry (GC‐MS) in full‐scan mode. The parent drug androst‐4‐ene‐3,6,17‐trione and androst‐4‐ene‐6 α ,17 β ‐diol‐3‐one and androst‐4‐ene‐6 α ‐ol‐3,17‐dione were detected in the post‐administration urine samples. Because androst‐4‐ene‐3,6,17‐trione is an anabolic steroid and an aromatase inhibitor, this substance is regarded as a doping agent. Hence, a selective and sensitive GC‐MS method in selected ion monitoring mode for the detection of the TMS‐enol‐TMS‐ether derivatives of these substances was developed and validated for doping control purposes. The limit of detection (LOD) of the investigated compounds ranged from 5 to 10 ng[sol ]mL. Using this method, the detection time for androst‐4‐ene‐3,6,17‐trione and androst‐4‐ene‐6 α ,17 β ‐diol‐3‐one was 24 h, while androst‐4‐ene‐6 α ‐ol‐3,17‐dione could be detected up to 37 h after administration of the dose recommended by the manufacturer. Copyright © 2005 John Wiley & Sons, Ltd.