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Quantitative analysis and pharmacokinetic comparison of multiple bioactive components in rat plasma after oral administration of Qi‐Shen‐Ke‐Li formula and its single‐herb extracts using ultra‐high‐performance liquid chromatography–tandem mass spectrometry
Author(s) -
Zhou Hui,
He Yang,
Zheng Zhong,
Liu Zhiqiang,
Song Fengrui,
Liu Shu
Publication year - 2020
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4959
Subject(s) - chemistry , pharmacokinetics , herb , medicinal herbs , chromatography , oral administration , chinese herbs , tandem mass spectrometry , herbal extracts , pharmacology , traditional medicine , mass spectrometry , traditional chinese medicine , medicine , alternative medicine , pathology
Qi‐Shen‐Ke‐Li (QSKL), a traditional Chinese formula prepared from six herbs, has long been used for the treatment of coronary heart disease and chronic heart failure. However, the herbal combination mechanism and underlying material basis of this multi‐herbal formula are not clear. In this study, an ultra‐high‐performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method to simultaneously determine multiple bioactive compounds in QSKL was established and validated. Using the developed method, 18 bioactive components in rat plasma after oral administration of QSKL formula and its single herb extracts were quantified. Based on these results, pharmacokinetic (PK) parameters ( T 1/2 , T max , C max , AUC 0–48h , and AUC 0–∞ ) of the 18 bioactive components were analyzed and compared using PKSlover 2.0 PK software. The experimental data suggested that significant changes in PK profiles were observed between the QSKL formula and its single‐herb extracts. The herbal combination in QSKL significantly influences the system exposure and the PK behaviors of the 18 bioactive components, indicating multicomponent interactions among the herbs. This study provides insight into the herbal combination mechanism and underlying material basis of the QSKL formula.