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Accurate and simple determination of oxcarbazepine in human plasma and urine samples using switchable‐hydrophilicity solvent in GC–MS
Author(s) -
Erarpat Sezin,
Bodur Süleyman,
Ayyıldız Merve Fırat,
Günkara Ömer Tahir,
Erulaş Fatih,
Chormey Dotse Selali,
Turak Fatma,
Budak Türkan Börklü,
Bakırdere Sezgin
Publication year - 2020
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4915
Subject(s) - chemistry , chromatography , solvent , detection limit , analyte , calibration curve , oxcarbazepine , gas chromatography–mass spectrometry , mass spectrometry , analytical chemistry (journal) , organic chemistry , carbamazepine , neuroscience , epilepsy , biology
Abstract This work presents a sensitive and rapid analytical method for the determination of oxcarbazepine in human plasma and urine samples. A vortex‐assisted switchable hydrophilicity solvent‐based liquid phase microextraction (VA–SHS–LPME) was used to preconcentrate oxcarbazepine from the samples before the determination by gas chromatography mass spectrometry. The switchable hydrophilicity solvent was synthesized by protonating N , N ‐dimethylbenzylamine with carbon dioxide to make it totally miscible with an equivalent volume of water. Parameters of the VA–SHS–LPME method including volume of switchable hydrophilicity solvent, concentration/volume of sodium hydroxide and vortex period were systematically optimized. Under the optimum conditions, good linearity ranging from 27.03 to 353.47 μg/kg was obtained for the analyte. Limit of detection and quantitation values were found to be 6.2 and 21 μg/kg (mass base), respectively. The relative standard deviation was calculated as 6.9% for six replicate measurements of the lowest concentration of the calibration plot. Satisfactory recovery results were calculated in the range of 97–100% for human plasma and urine samples spiked at five different concentrations.