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Determination of plasma homocysteine with a UHPLC–MS/MS method: Application to analyze the correlation between plasma homocysteine and whole blood 5‐methyltetrahydrofolate in healthy volunteers
Author(s) -
Ji Yubin,
Luo Hongjun,
Li Hui,
Lin Zhexuan,
Luo Wenhong
Publication year - 2020
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4845
Subject(s) - chemistry , chromatography , homocysteine , derivatization , detection limit , extraction (chemistry) , whole blood , solid phase extraction , mass spectrometry , biochemistry , medicine
An ultra‐high performance liquid chromatography tandem mass spectrometry method was developed for determination of homocysteine (HCY) in human plasma. The HCY was derivatized with 2‐chloro‐1‐methylquinolinium tetrafluoroborate and isolated using solid‐phase extraction. Derivatization, isolation and detection procedures were optimized. Satisfactory linearity was obtained with determination coefficients ( r 2 ) >0.999. The intra‐ and inter‐day precisions were in the interval of 1.2–5.1% and accuracy was within ±7%. Mean recoveries were close to 100%. The limit of detection and the limit of quantification were 0.46 and 1.38 μmol/L, respectively. The method was then applied to investigate the relationship between plasma HCY and whole blood 5‐methyltetrahydrofolate levels in healthy volunteers. The results revealed that the plasma level of HCY was significantly negatively correlated to whole blood 5‐methyltetrahydrofolate in volunteers.