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Activated carbon/diatomite‐based magnetic nanocomposites for magnetic solid‐phase extraction of S ‐phenylmercapturic acid from human urine
Author(s) -
Shan Xiaoyue,
Tan Siwei,
Shi Yanpeng,
Shao Ji,
Su Kewen,
Zhang Ling,
Feng Hui,
Ye Haipeng
Publication year - 2020
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4834
Subject(s) - chemistry , elution , adsorption , solid phase extraction , chromatography , extraction (chemistry) , activated carbon , fourier transform infrared spectroscopy , enrichment factor , analytical chemistry (journal) , desorption , detection limit , solvent , scanning electron microscope , materials science , chemical engineering , composite material , organic chemistry , engineering
In this study, activated carbon/diatomite‐based magnetic nancomposites (denoted as AC/DBMNs) were synthesized and applied as an adsorbent for magnetic solid‐phase extraction of S‐ phenylmercapturic acid ( S‐ PMA) from human urine prior to high‐performance liquid chromatography. The surface morphologies and structures of AC/DBMNs were characterized by Fourier transform infrared spectroscopy, transmission and scanning electron microscopy, X‐ray diffraction, Brunauer–Emmett–Teller surface area, vibrating sample magnetometer and ζ ‐potential measurements. The experimental parameters including sample volume, sample pH, adsorbent amount, extraction time, elution solvent and desorption time were investigated in detail. Under the optimum conditions, the method exhibited good linearity ( r > 0.9993) within the concentration ranges of 0.03–1.0 mg/L. Moreover, the limits of detection and quantification were 0.01 and 0.03 mg/L, respectively. The enrichment factor was 5, and good recoveries (88.9–97.3%) with relative standard deviations in the range of 5.6–6.8% ( n = 6) for inter‐day and 6.3–8.1% ( n = 6) for intra‐day were achieved. The developed method was successfully applied to the analysis of S‐ PMA in urine samples. In addition, this accurate and sensitive method has great potential to be applied in the early screening and clinical diagnosis of the workers exposed to benzene.